Digital Imaging

Digital Imaging FacilityThe Digital Imaging division provides whole-slide histologic imaging suitable to prepare publication-quality figures and quantization of selected features from digitized images.

For IHC stain quantization, an Aperio ScanScope XT digital slide scanner is used to prepare diagnostic quality 20X or 40X magnification images, and a Wacom Cintiq 21UX pen tablet screen is used for image annotation. Representative regions (typically in quadruplicate) within stained sections are annotated. Aperio software tools such as Positive Pixel Count are used to compute the average pixel intensity (in a colorimetric channel corresponding to the brown IHC precipitate) of all pixels within each annotation region.

The Digital Imaging division is located in rooms C434 and C428 of the Mayo building and equipped with an Aperio ScanScope XT digital slide scanner, a dedicated server equipped with Aperio image analysis algorithm framework software, and a Dell Optiplex 755 computer equipped with a Wacom Cintiq 21UX pen tablet screen. The laboratory is equipped with sophisticated image analysis software packages including ImagePro Plus 6.0, Adobe Photoshop CS3, and Aperio applications including Positive Pixel Count, Area Quantification, Cell Quantification, and Microvessel Analysis. The Aperio applications employ smart object recognition algorithms for semi-automated and standardized characterization of specific histologic features. Positive Pixel Count and Area Quantification algorithms are useful to quantify epithelial and stromal features, including quantification of immunohistochemical stains.